Feeder-free culture of human embryonic stem cell line BG01V/hOG using magnetic field-magnetic nanoparticles system.
Biomed Pharmacother
; 67(1): 17-21, 2013 Feb.
Article
in En
| MEDLINE
| ID: mdl-23089476
ABSTRACT
PURPOSE:
Human embryonic stem (hES) cells are useful tools for regenerative medicine. Maintaining hES cells for research and clinical purposes remains a challenge. The hES cells have typically been grown on a mouse or human cell feeder layer, but these methods harbor potential health problems for the recipient. A culture system using magnetic field and iron oxide nanoparticles were previously demonstrated to sustain mouse embryonic stem cells in vitro. Now, by using the BG01v/hOG cell line, we could assess the effect of this culture system on the stemness of an embryonic stem cell of human origin.METHODS:
Using a variant hES cell line, BG01V/hOG, expressing an emerald green fluorescent protein (EmGFP), we grown these cells in the presence of serum-free medium supplemented with magnetic nanoparticles functionalized with citrate. The cells were positioned over a circular magnet (4000 Gauss) and monitored daily by fluorescence microscopy.RESULTS:
We discovered that hES cells can proliferate when labeled with magnetic nanoparticles and in the presence of a magnetic field without losing pluripotency.CONCLUSION:
These results establish an alternative method for maintaining hES cells which would minimize health concerns as well as label cells for subsequent clinical tracking.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Culture Techniques
/
Pluripotent Stem Cells
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Embryonic Stem Cells
/
Magnetite Nanoparticles
Limits:
Animals
/
Humans
Language:
En
Journal:
Biomed Pharmacother
Year:
2013
Document type:
Article
Affiliation country: